Heterokaryosis and you may parasexual recombination into the pathogenic strains out-of Fusarium oxysponrm
V. Heterokaryosis and you may parasexuality
Utilize the “0”location for one of the biological parents and you will note the tension amount toward dish. Use the theme toward replicator. Incubate dos-3 days. Simulate the segregants into the some sample dishes using good replicator with, age.grams., 21 needles. Mark the new dishes that have a variety. Incubate 2-3 days. Get the test plates and you will checklist brand new phenotypes regarding scoring table. Make an effort to influence the ploidy of one’s colonies on the base from the latest markers. Browse the ploidy away from uncertain territories. Create a list of the new genotypes (you are able to a utility). Influence the brand new part of the recombinants with the various other indicators. Hence markers was linked? Could you select intrachromosomal recombination? Where linkage class ‘s the unknown marker?
Contained in this experiment i influence the new gene acquisition and you may area of this new centromere during the linkage classification VI ofA. niger.Various strategies for the selection of mitotic recombinants are utilized. The fresh new markers in it is: pubA1, pyrB4, c d l . The brand new c d locus is actually critical with the chromosome sleeve and thus really suitable since the selection marker. As the all markers is actually recessive, they must be during the cis position. The chlorate-resistant segregants is isolated, plus they feel examined into the almost every other indicators. (más…)
